BLIRT ExtractMe Plasmid DNA 96-well Kit
High-throughput extraction of plasmid DNA in 96-well format
|Product||Cat No.||Pack Size|
|ExtractMe Plasmid DNA 96-well Kit (centrifuge)||SWA- EM21-192||2x 96 preps|
|SWA-EM21-960||10x 96 preps|
|ExtractMe Plasmid DNA 96-well Kit (centrifuge or vacuum)||SWA-EM23-192||2x 96 preps|
|SWA-EM23-960||10x 96 preps|
The EXTRACTME PLASMID DNA 96-WELL kit is designed for high-throughput and efficient purification of high quality plasmid DNA from recombinant Escherichia coli strains. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
0.5-10 ml bacterial culture per well
5-20 µg pDNA per well
40 µg DNA per well
55 minutes per plate
A260/A280 ratio = 1.7 – 1.9
standard SBS footprint
- Centrifuge with rotor for plates (≥3k x g);
- Automatic Liquid Handling Systems (eg. Biomek® FX, TECAN Freedom Evo, TECAN Genesis, Eppendorf epMotion)
The DNA purification procedure utilizes spin 96-minicolumns plates with membranes which efficiently and selectively bind nucleic acids. In the first isolation step, the plasmid DNA is released from bacterial cells by alkaline lysis. Then the lysate is neutralized and all the cell residues along with the proteins and genomic DNA are separated in the centrifugation step. The lysate is applied to the purification minicolumn membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified plasmid DNA is eluted using a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.
The quality of each production batch (LOT) of the EXTRACTME PLASMID DNA 96-WELL kit is tested using BLIRT’s ISO-certified quality management system. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometry. In addition, the functional quality is tested by qPCR, digestion with restriction enzymes and pDNA transfection.